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4876 ELISA LTI

Code and name of test:

4876 – the enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of antibodies against the avian infectious laryngotracheitis (ILT) virus

Avian infectious laryngotracheitis (ILT) is an avian respiratory disease caused by a herpesvirus, with widespread distribution, which affects poultry and wild birds. According to the OIE Manual, the laboratory diagnosis of this disease is based on proving the presence of the virus or viral antigens or on proving the presence of the specific antibodies in the bird serum. The etiologic agent only affects birds and has no significance in human pathology.

Palliative treatments in the outbreaks of disease, with repeated sets of antibiotics and vitamins, primarily aim at reducing mortality losses due to secondary infections with other germs.

Disease control is accomplished by appropriate vaccinations, carried out in the 6th and 14th week, respectively; their effectiveness can be serologically tested by way of ELISA.

The serological exam seeks to detect the specific antibodies of the avian infectious laryngotracheitis virus by using ELISA immunoenzymatic techniques that meet the requirements of specificity and sensitivity.

Usefulness:

  • Monitoring the poultry flocks in terms of the status of the infection with the avian infectious laryngotracheitis virus
  • Monitoring the poultry flocks in terms of the results of the specific vaccinations
  • A tool in preventing the spread of ILT within and between consignments of birds
  • Monitoring the safety of the obtained biological products on birds

Bibliography:

  • Daniela Botus, Virgilia Popa, O. Radulescu, Jenica Bucur, 2010, Serological data of avian infectious diseases during 2009, St. Med. Vet. USAMV Timisoara, vol. XLIII(1), 187-197
  • Daniela Botus, Virgilia Popa, E. Caplan, F. Pastrama, M. Pirvulescu, 2010, Validation of an immunoenzymatic assay for detection of antibodies against avian infectious laryngotracheitis virus, St. Med. Vet. USAMV Timisoara, vol. XLIII(1), 197-204
  • Mihailescu R., Botus D, Popa V., 2007, Evaluating the immune response against Egg drop Syndrome (EDS) and Infectious Laryngotracheitis virus (ILT) by Enzyme Linked Immunosorbent Assay, Proceedings of the International Conference “Research people and actual tasks on multidisciplinary sciences”, 1, 215-219
  • Botus D., Mihailescu R., Popa V., Bucur J., 2006, ELISA kit for quantitative detection of antibody against Infectious Laryngotracheitis virus (ILT), Şt. Med. Vet., Univ. de Vest a Banatului, vol. XXXIX (USAMV Timisoara), 295-301
  • Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Ed 2013, cap. 2.3.3

Specimens:

gallinaceous bird blood serum

Response time:

2 business days

Methodology:

quantitative determination of the antibodies against the avian infectious laryngotracheitis virus from gallinaceous bird sera by way of the enzyme-linked immunosorbent ELISA assay, the indirect version

Reference values:

N/A