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4695 ELISA IBV

Code and name of test:

4695 – the enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of antibodies against the avian infectious bronchitis virus (IBV)

Infectious bronchitis (IB) is an acute, highly contagious disease of chickens characterized by respiratory signs (wheezing, sneezing, coughing, discharges from the nose), severe renal disease associated with nephrotropic strains, and significant decrease in egg production. IB is normally only encountered in hens. All age groups are susceptible, assuming that they were not previously exposed to the virus and there is no passive immunity.

There is no effective treatment against IB. For small chickens increasing the temperature in the shed or giving warm moist feed and correcting any visible management deficiencies can help.

The poultry can be immunized through suitable vaccination. For prevention for small chickens modified live vaccines are used.

Vaccines are effective if they contain the appropriate serotype for a certain epidemiological area. If they are administered to chickens with parenteral immunity, the vaccination should be repeated at least once. The effectiveness of the vaccination can be controlled serologically by enzyme-linked immunosorbent assays.

The serological exam seeks to detect the specific antibodies against the avian infectious bronchitis virus by using ELISA techniques that meet the requirements of specificity and sensitivity. High antibody titres usually mean protection against the disease.

Usefulness:

  • Monitoring the poultry flocks in terms of the status of the avian infectious bronchitis virus infection
  • Monitoring the poultry flocks in terms of the results of the specific vaccinations
  • A tool in preventing the spread of the avian infectious bronchitis virus within and between consignments of birds
  • Monitoring the safety of the obtained biological products on birds

Bibliography:

  • Daniela Botus, Virgilia Popa, O. Radulescu, Jenica Bucur, 2010, Serological data of avian infectious diseases during 2009, St. Med. Vet. USAMV Timisoara, vol. XLIII(1), 187-197
  • Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Ed 2013, cap. 2.3.2

Specimens:

gallinaceous bird serum

Response time:

2 business days

Methodology: quantitative determination of the antibodies against the avian infectious bronchitis virus from gallinaceous bird sera by way of the immunoenzymatic ELISA assay, the indirect version

Reference values:

N/A