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4692 IHA NDV

Code and name of test:

4692 -hemagglutination inhibition (HI) method for the diagnosis of the Newcastle disease

The Newcastle disease is widely spread and affects many types of birds, both poultry and wild birds, and is characterized by various morbidity, mortality, signs and lesions. All age groups are susceptible to this disease; people who come into contact with the Newcastle disease virus for the first time may also develop mild conjunctivitis.

The effectiveness of vaccination can be monitored serologically by the reaction of hemagglutination inhibition (antibody detection). The higher the antibody titres of a vaccinated flock are, the higher the protection against the disease is. Higher antibody titres also indicate protection in the case of hatched chickens with maternal antibodies.

Usefulness:

  • Detecting and determining the titre of antibodies against the Newcastle disease virus
  • Assessing the immune status of the tested flocks
  • - Monitoring the safety of the obtained biological products on birds

Bibliography:

  • - Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Ed 2013, chapter 2.3.14

Specimens:

bird blood serum (hen, turkey, pigeon)

Response time:

2 business days

Methodology:

determination of the titre of antibodies against the Newcastle disease virus from the bird blood serum by the reaction of hemagglutination inhibition (HI) – micromethod.

Reference values:

Value of HI titre (TIHA) TIHA ≤ 4 Negative
TIHA =  8 Suspicious
TIHA ≥ 16 Positive