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Instructions for collecting samples

Samples collected on swabs:

  • For bacteriological tests, the samples will be collected on swabs with an AMIES transport medium
  •  For sanitation tests, the samples will be collected on simple sterile swabs (without a transport medium)
  •  For PCR tests, the samples will be collected on simple sterile swabs (without a transport medium)

Solid samples / swabs:

The solid samples of faeces, the faecal / cloacal / rectal / respiratory swabs, fresh tissue samples and other samples without liquids are introduced in individual containers that are clean / sterile, individually labelled, without a transport medium and well closed / sealed, such as ziplock bags or simple tubes, which are also introduced in an envelope. If the primary containers are larger, these shall be placed in a box instead of an envelope.

If more samples collected on swabs (i.e. swabs from the throat / pharyngeal swabs / nasal swabs / swabs with ocular discharges) are required for a sole panel or test, all swabs collected from the same animal will be introduced in a single labelled bag or a single tube.

The swabs (the simple ones and those with an AMIES transport medium) for the sanitation and bacteriological examinations will be delivered at ambient temperature, within maximum 5 days.

The other types of solid samples and the swabs for the PCR tests, kept at room temperature (15°C – 25°C), will be delivered, if possible, on the same day on which they were collected.

If the samples must be kept for longer than a few hours before delivery, then they will be refrigerated (2°C – 8°C) and delivered in a few days’ time, in insulated bags/cases or in packages with gel packs (“penguins”, pre-frozen), which ensure a low temperature (8°C – 15°C). The temperature must not exceed 25°C during transportation.

The fixed or preserved tissues may be transported at ambient temperature.

 Liquid samples (for PCR, bacteriological, biochemical tests)

The fully fresh blood samples are collected in tubes with EDTA or ACD, which are then sealed and agitated by canting several time, so that the preservation substance is mixed in with the blood.

Other liquid samples (liquid for lavage, cerebrospinal fluid, serum samples, with the last ones also being intended for the ELISA tests) are collected in simple, clean/sterile and sealed tubes or containers.

If possible, the liquid samples will be delivered by courier services on the same day on which they were collected. If the samples must be kept for longer than a few hours before the delivery to the laboratory, then they will be refrigerated. Generally, the liquid samples may be transported at room temperature (15°C – 25°C); however, if the transport takes longer than 24 hours or occurs in periods of hot weather, the liquid samples will be transported in a cold environment, in an insulated bag/case with pre-frozen gel packs, in a well closed package which is pressure or crushing resistant.

Frozen samples (for PCR tests)

The frozen samples will be transported in such a manner that they remain frozen until their arrival in the laboratory. They will not be defrosted prior to the delivery. The laboratory must be alerted if the transport of the frozen samples takes longer than 24 hours.

Dry card samples

Our laboratory may perform PCR tests on blood samples and other liquids collected on dry cards. The liquid samples are placed on the card and are dried according to the producer instructions / as provided on the back of the card. The card samples will remain stable, for several weeks, at room temperature.

The samples of fodder and feed materials for the mycotoxicological, mycological and bacteriological examinations will be transported in bags or recipients for 500 g of product, well closed and transport resistant.

Sample labelling

Each sample / sample recipient will be uniquely and clearly labelled with an identification code (ID) of the sender’s choice. This code will be found in the Laboratory test report and in the sample Waybill, filled in by the sender.

Secondary packaging

All primary recipients (tubes, bags, etc.) shall be introduced in a sealed secondary container (a ziplock bag or another sealed container). The glass tubes must be protected from each other, so that they do not break during transport. Absorbent materials will be placed in the secondary packaging (paper, towel, etc.), in order to fully absorb the sample liquids and thus to prevent the environment contamination in case of possible accidents during the transport. Furthermore, separators / dunnages (fardages) will be used for the protection and stability of primary packages. The transport container will be labelled in compliance with the requirements of the transport company.

A Waybill filled in by the sender will be included in each secondary transport container.

Sample takeover schedule

Our laboratory receives sample deliveries every Monday to Friday, between 8:30 and 16:00 hours.

If a weekend delivery is required, please contact us at least 24 hours in advance, in order to confirm the receipt of samples over the weekend.

All samples will be sent to the address:

Pasteur Institute
Diagnosis Centre – PIEBA office (receipt, sample registration, issue of laboratory reports)
333 Calea Giulesti, district 6, Bucharest, Postal code 060269
Information and contact details: Dr. Marian Culcescu
mobile phone: 0740703797
telefax: 021 2219960 – extension 1122
e-mail: secretariat@farmavet.ro